Composite

Part:BBa_K4260006:Design

Designed by: Claudia Angélica García Alonso   Group: iGEM22_TecCEM   (2022-10-12)


Isoeugenol Monooxygenase, inducible coding sequence: Trp term, IemR, promoter, pelB, Iso & rrnBT1 te


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 439
    Illegal XhoI site found at 267
    Illegal XhoI site found at 294
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1187
    Illegal NgoMIV site found at 1895
    Illegal NgoMIV site found at 2503
    Illegal AgeI site found at 1719
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1123
    Illegal BsaI.rc site found at 1815


Design Notes

Since the coding sequence of IemR was in the antisense chain, the reverse-complementary sequence of the Trp terminator was used, in order to stop the transcription of the IemR.


Source

The transcriptional activator (IemR) and the promoter, come from Pseudomonas nitroreducens Jin1. The Isoeugenol monooxygenase comes from Pseudomonas putida IE27.

References